gWiz™ High Expression Reporter Plasmids

The gWiz plasmids are designed for the highest level of gene expression in a wide range of mammalian cells and tissues. They contain a proprietary modified promoter, followed by intron A from the CMV immediate early gene, and a high-efficiency artificial transcription terminator. gWiz expression vectors are constructed using a plasmid backbone that has been extensively modified to achieve enhanced levels of transgene expression in mammalian cells.

Aldevron's exclusive fermentation technology and DNA purification process are used to produce ultrapure, milligram amounts of the gWiz™ plasmids. These ready-to-use plasmids are endotoxin-free and can be applied directly to most in vitro and in vivo applications. Each gWiz plasmid is delivered in the buffer of your choice with delivery time of approximately 24 to 48 hours. Volume discounts are available. Common features of the gWiz plasmids include:

In vitro or in vivo gene expression
CMV IE promoter
Intron A
Artificial transcription terminator
Kanamycin resistance

gWiz high expression reporter plasmids available from Aldevron.

<table class="table_order"><thead><tr><th>Vector</th><th>Cat #</th><th>Price</th><th>Order</th></tr></thead><tbody><tr><td class="labelcell"><strong class="item_title">gWiz-GFP</strong><p><a href="https://606687.secure.netsuite.com/core/media/media.nl?id=1594&c=606687&h=4f1ad11f91d7b22e1c9a" target="_blank" class="button zoom-map cboxElement" rel="noopener" onclick='_gaq.push(["_trackEvent","Button","Map","5006/5007 gWiz-GFP"])'>MAP</a></p><div class="pricetable mmg-collapsible"><strong class="module-title">QUANTITY PRICING</strong><div id="pricetable_56373" class="content" style="display:none">Cat# 5007 Only<table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1080.00</td></tr><tr><td>2</td><td>$935.00</td></tr><tr><td>3</td><td>$860.00</td></tr><tr><td>4</td><td>$845.00</td></tr><tr><td>5+</td><td>$804.00</td></tr></tbody></table></div></div><table class="table_orderNested"><tbody><tr><td><span class="info_label">Transgene</span></td><td>Green Fluorescent Protein</td></tr><tr><td><span class="info_label">Plasmid Size</span></td><td>5757 bp</td></tr><tr><td><span class="info_label">Notes</span></td><td>GFP expression in mammalian cells and tissues</td></tr></tbody></table></td><td>5006<br>5007</td><td><span class="price" id="price_56482">$590.00</span>- 5 mg<br><span class="price" id="price_56373">$1,080.00</span>- 10 mg</td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/dnas/gwiz" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" name="buyid"><option value="56482">5 mg</option><option value="56373">10 mg</option></select><br><button type="submit" class="addtocart" onclick='trackCart("5006/5007 gWiz-GFP",this.parentNode.elements.buyid,this.parentNode.elements.qty.value)'>Add to Cart</button></form></td></tr><tr><td class="labelcell"><strong class="item_title">gWiz-Luc</strong><p><a href="https://606687.secure.netsuite.com/core/media/media.nl?id=706&c=606687&h=38863950c295c63ad752" target="_blank" class="button zoom-map cboxElement" rel="noopener" onclick='_gaq.push(["_trackEvent","Button","Map","5000/5001 gWiz-Luc"])'>MAP</a></p><div class="pricetable mmg-collapsible"><strong class="module-title">QUANTITY PRICING</strong><div id="pricetable_56370" class="content" style="display:none">Cat# 5001 Only<table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1080.00</td></tr><tr><td>2</td><td>$935.00</td></tr><tr><td>3</td><td>$860.00</td></tr><tr><td>4</td><td>$845.00</td></tr><tr><td>5+</td><td>$804.00</td></tr></tbody></table></div></div><table class="table_orderNested"><tbody><tr><td><span class="info_label">Transgene</span></td><td>Luciferase</td></tr><tr><td><span class="info_label">Plasmid Size</span></td><td>6732 bp</td></tr><tr><td><span class="info_label">Notes</span></td><td>Luc expression in mammalian cells and tissue</td></tr></tbody></table></td><td>5000<br>5001</td><td><span class="price" id="price_56479">$590.00</span>- 5 mg<br><span class="price" id="price_56370">$1,080.00</span>- 10 mg</td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/dnas/gwiz" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" name="buyid"><option value="56479">5 mg</option><option value="56370">10 mg</option></select><br><button type="submit" class="addtocart" onclick='trackCart("5000/5001 gWiz-Luc",this.parentNode.elements.buyid,this.parentNode.elements.qty.value)'>Add to Cart</button></form></td></tr><tr><td class="labelcell"><strong class="item_title">gWiz-blank</strong><p><a href="https://606687.secure.netsuite.com/core/media/media.nl?id=1592&c=606687&h=9c668159252a11acd146" target="_blank" class="button zoom-map cboxElement" rel="noopener" onclick='_gaq.push(["_trackEvent","Button","Map","5008/5009 gWiz-blank"])'>MAP</a></p><div class="pricetable mmg-collapsible"><strong class="module-title">QUANTITY PRICING</strong><div id="pricetable_56374" class="content" style="display:none">Cat# 5009 Only<table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1080.00</td></tr><tr><td>2</td><td>$935.00</td></tr><tr><td>3</td><td>$860.00</td></tr><tr><td>4</td><td>$845.00</td></tr><tr><td>5+</td><td>$804.00</td></tr></tbody></table></div></div><table class="table_orderNested"><tbody><tr><td><span class="info_label">Transgene</span></td><td>none</td></tr><tr><td><span class="info_label">Plasmid Size</span></td><td>5100 bp</td></tr><tr><td><span class="info_label">Notes</span></td><td>Multiple cloning site for inserting gene of interest; Expression in mammalian cells and tissue</td></tr></tbody></table></td><td>5008<br>5009</td><td><span class="price" id="price_56483">$590.00</span>- 5 mg<br><span class="price" id="price_56374">$1,080.00</span>- 10 mg</td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/dnas/gwiz" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" name="buyid"><option value="56483">5 mg</option><option value="56374">10 mg</option></select><br><button type="submit" class="addtocart" onclick='trackCart("5008/5009 gWiz-blank",this.parentNode.elements.buyid,this.parentNode.elements.qty.value)'>Add to Cart</button></form></td></tr></tbody></table>

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gWiz Quality Control Specifications

Appearance	Clear and colorless
Buffer	Water
Concentration	5.0 mg/mL +/- 10%
DNA Homogeneity	Predominantly supercoiled
Endotoxin	≤ 100 EU/mg
Identity	Confirmed Against DNA Ladder
ABS 260/280 Ratio Purity	1.80 - 2.00
Residual Host Genomic DNA	≤ 5%
Residual Host RNA	Not visible at 200 ng load

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