Spy FiTM Cas9 Nuclease
Reduced off-target activity without loss of editing efficiency
From basic research to clinical applications, our SpyFi Cas9 Nuclease delivers greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. SpyFi Cas9 Nucleases are available at Research-Grade, GMP-Source™ and cGMP levels.
SpyFi Cas9 Nuclease is uniquely positioned to satisfy a wide range of needs from basic research to clinical applications. When used in combination with effective guide, target site and double-stranded DNA cut strategies, SpyFi Cas9 Nuclease delivers greater editing efficiency, higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Functional Performance
- Reduced off-target effects
- Ready to use for electroporation or transfection
- 10 mg/mL formulation for ease of use
Manufacturing and Quality Control
- Research-grade to cGMP
- Consistent activity and purity across all lots
Aldevron’s SpyFi Cas9 Nuclease is sold under license of patents and/or patents pending from Integrated DNA Technologies, Inc. (IDT). Data shown above was generated in collaboration with IDT.
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| Purity, RP-HPLC | ≥95% |
| Purity, SDS-PAGE | ≥95% |
| Aggregation | ≤5% aggregate |
| Concentration | 10.0 mg/mL +/- 10% |
| Endotoxin | ≤ 10 EU/mg |
| Residual DNase | < LOQ |
| Residual RNase | < LOQ |
| Appearance | Clear, colorless |
| Residual Host Protein | ≤ 200 ng/mL |
| Residual Host GenomicDNA | < 1% (w/w) |
| Activity Assay, in vitro | ≥80% of uncut reference DNA converted to cleavage products |
| Bioburden | < 2 CFU/Sample |
| Mycoplasma | Negative for the presence of Mycoplasma |
Ordering Information
| Product | Small Size | Catalog Number | Large Sizes | Catalog Number |
| SpyFi™ Cas9 Nuclease | 0.25MG | 9214-0.25MG | 5MG | 9214-5MG |
| cGMP-Manufactured Nucleases | ||||
| SpyFi™ Cas9 Nuclease – GMP | 1MG | 9216-0.1ML | 10MG | 9216-1ML |
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WHITEPAPER: Next-generation CRISPR Approaches
CASE STUDY: Enabling Novel CRISPR-Cas9 Delivery
