Codex® HiCap RNA Polymerase
in vitro transcription for advancing RNA medicine
Codex® HiCap RNA Polymerase is a co-transcriptional capping RNA polymerase engineered to produce synthetic RNA to meet increasing demands for safety and cost-effectiveness. The HiCap enzyme generates fewer undesirable double-stranded RNA (dsRNA) byproducts than wild-type T7 (WT T7) RNA polymerase, reducing unwanted immunogenicity, while efficiently incorporating commercially available cap analogs.
dsRNA byproducts
Figure 1. Significantly reduced dsRNA byproducts by Codex® HiCap RNA Polymerase. mRNAs were transcribed using either WT T7 or Codex® HiCap RNA Polymerase. After IVT was completed, total dsRNA content was quantified via sandwich ELISA using the J2 dsRNA antibody.
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Codex® HiCap RNA Polymerase vs. Wild-type T7
| Codex® HiCap RNA Polymerase | Wild-type T7 polymerase | |
| Reduced dsRNA Production | +++ | + |
| Co-transcriptional capping efficiency | +++ | ++ |
| IVT yields | +++ | +++ |
| Manufacturing simplification | +++ | + |
| Finished Product Yield | +++ | + |
Codex® HiCap RNA Polymerase Resources
- Codex® HiCap RNA Polymerase Brochure
- Codex® HiCap RNA Polymerase in vitro Transcription Procedure
- Codex® HiCap RNA Polymerase Guide: Maximizing mRNA Titers
- Poster: HiCap T7 transcribes RNA with higher purity and activity while using less cap analog
- Research Article: An Engineered T7 RNA Polymerase for efficient co-transcriptional capping with reduced dsRNA byproducts in mRNA synthesis
- Press Release: Codexis and Aldevron Enter Exclusive Licensing Agreement for Codex® HiCap RNA Polymerase
Ordering Information
| Catalog # | Product Description | Size |
| 9145-250 µL | Codex® HiCap RNA Polymerase | 250 µl |
| 9145-1mL | Codex® HiCap RNA Polymerase | 1 ml |
| 9145-10mL | Codex® HiCap RNA Polymerase | 10 ml |
*Codex® is a trademark of Codexis, Inc. Any other third-party trademarks are the property of their respective owners.
