pALD-HELP for rAAV Production

In Stock, Royalty-Free, Helper Plasmid Solution for Recombinant AAV Manufacturing

Triple transfection is the most common method for rAAV production and does not require co-infection with adenovirus; a helper plasmid provides adenoviral genes. Aldevron's pALD-HELP has several advantages:

Immediately available at research grade and GMP-Source®
Royalty free, including for commercial products
Replaces any rAAV helper plasmid
Kanamycin resistance
Consistent, reliable supply

With Aldevron's pALD-HELP, clients do not need to manufacture a custom batch of helper plasmid because Aldevron maintains it as an in-stock product, providing a high-quality and consistent source, helping reduce timelines and cost.

Available Pack Sizes

Product	Free Sample	Research Grade	GMP-Source®	cGMP
pALD-HELP	100 μg	10 mg	10 mg	Custom Request

pALD-HELP, pALD-AAV Rep/Cap Infectious Titer Data

Infectious titer data for rAAV vector production in adherent HEK293T cells using pALD-HELP and pALD-AAV rep/cap plasmids as compared to commercially available rep/cap plasmids.

<table class="table_order"><thead><tr><th>Description</th><th>Cat #</th><th>Price</th><th>Order</th></tr></thead><tbody><tr><td><p><strong class="item_title">pALD-HELP RG 10mg</strong></p></td><td>5082-10</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">5082-10 10mg Pricing</strong><div id="pricetable_184439" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1790.00</td></tr><tr><td>2</td><td>$1360.00</td></tr><tr><td>3</td><td>$1220.00</td></tr><tr><td>4</td><td>$1150.00</td></tr><tr><td>5+</td><td>$1106.00</td></tr></tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/pald-aav/pald-help" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="184439">10 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr></tbody></table>

Additional discounts available for quantities > 5 vials.

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pALD-HELP Quality Control Specifications

Appearance	Clear and Colorless
Buffer	TE
USP Sterility <71> *	No Growth
Concentration	1.0 mg/mL +/- 10%
DNA Homogeneity	≥ 80% Supercoiled
Endotoxin	≤ 100 EU/mg
Identity	Confirmed Against DNA Ladder
Plasmid Identity	Identical to Reference Sequence
ABS 260/280 Ratio Purity	1.80 - 2.00
Residual Host Genomic DNA via qPCR	≤ 5%
Residual Host Protein via BCA	≤ 2%
Residual Host RNA via SYBR Gold	≤ 5% by Semi-Quantitative Densitometry
Restriction Digest	Matches Expected Restriction Pattern
Mycoplasma Contamination	Negative for the Presence of Mycoplasma

*Sterility testing is not available for research grade material. Bioburden by direct inoculation with a specification of no growth after 48 hours is performed at the research grade level.

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Additional Resources

Comparison of pALD-HELP and pALD-X80 (PDF)

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