pALD-HELP for rAAV Production
In Stock, Royalty-Free, Helper Plasmid Solution for Recombinant AAV Manufacturing
Triple transfection is the most common method for rAAV production and does not require co-infection with adenovirus; a helper plasmid provides adenoviral genes. Aldevron's pALD-HELP has several advantages:
- Immediately available at research grade and GMP-Source®
- Royalty free, including for commercial products
- Replaces any rAAV helper plasmid
- Kanamycin resistance
- Consistent, reliable supply
With Aldevron's pALD-HELP, clients do not need to manufacture a custom batch of helper plasmid because Aldevron maintains it as an in-stock product, providing a high-quality and consistent source, helping reduce timelines and cost.
| Product | Free Sample | Research Grade | GMP-Source® | cGMP |
| pALD-HELP | 100 μg | 10 mg | 10 mg | 10 mg |
Infectious titer data for rAAV vector production in adherent HEK293T cells using pALD-HELP and pALD-AAV rep/cap plasmids as compared to commercially available rep/cap plasmids.
<table class="table_order"><thead><tr><th>Description</th><th>Cat #</th><th>Price</th><th>Order</th></tr></thead><tbody><tr><td><p><strong class="item_title">pALD-HELP RG 10mg</strong></p></td><td>5082-10</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">5082-10 10mg Pricing</strong><div id="pricetable_184439" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody>
<tr>
<td>1-9</td>
<td>$810.00</td>
</tr>
<tr>
<td>10+</td>
<td>$675.00</td>
</tr>
</tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/pald-aav/pald-help" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="184439">10 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr></tbody></table>
Additional discounts available for quantities > 5 vials.
| Appearance | Clear and Colorless |
| Buffer | TE |
| USP Sterility <71> * | No Growth |
| Concentration | 1.0 mg/mL +/- 10% |
| DNA Homogeneity | ≥ 80% Supercoiled |
| Endotoxin | ≤ 100 EU/mg |
| Identity | Confirmed Against DNA Ladder |
| Plasmid Identity | Identical to Reference Sequence |
| ABS 260/280 Ratio Purity | 1.80 - 2.00 |
| Residual Host Genomic DNA via qPCR | ≤ 5% |
| Residual Host Protein via BCA | ≤ 2% |
| Residual Host RNA via SYBR Gold | ≤ 5% by Semi-Quantitative Densitometry |
| Restriction Digest | Matches Expected Restriction Pattern |
| Mycoplasma Contamination | Negative for the Presence of Mycoplasma |
*Sterility testing is not available for research grade material. Bioburden by direct inoculation with a specification of no growth after 48 hours is performed at the research grade level.