Rep/Cap Plasmids
In Stock, Royalty-Free, Packaging Plasmid Solution for Recombinant AAV Manufacturing
pALD-AAV Rep/Cap Plasmids
Recombinant AAV (rAAV) manufacturing is composed of four main parts which include a rep/cap plasmid, a helper plasmid, an expression plasmid, and a viral packaging cell line. Triple transfection of the three plasmids into HEK293 cells is the most frequently used rAAV production method.
Aldevron's pALD-AAV rep/cap plasmids have several advantages:
- Royalty free, including for commercial products
- Kanamycin resistance
- Consistent, reliable supply
- Immediately available at research grade
With Aldevron's rep/cap plasmids, clients do not need to manufacture a custom batch as Aldevron maintains each as an in-stock product, reducing timeline and cost.
Each rep/cap plasmid contains the gene sequence for AAV serotype 2 replication (rep) protein paired with either serotype 2, 5 or 6 capsid (cap) protein respectively. Use of AAV serotype 2 rep proteins in combination with other serotypes for cap proteins is referred to as pseudotyping which provides enhanced and refined tissue tropism in some applications.
| Plasmid | Rep Serotype | Cap Serotype | Pack Size |
| pALD-AAV2 | 2 | 2 | 10 mg |
| pALD-AAV5 | 2 | 5 | 10 mg |
| pALD-AAV6 | 2 | 6 | 10 mg |
Bulk quantities and samples are available upon request.
Plasmid Maps
<table class="table_order"><thead><tr><th>Description</th><th>Cat #</th><th>Price</th><th>Order</th></tr></thead><tbody><tr><td><p><strong class="item_title">pALD-AAV2</strong></p></td><td>5057-10</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">5057-10 10 mg Pricing</strong><div id="pricetable_145287" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody>
<tr>
<td>1-9</td>
<td>$810.00</td>
</tr>
<tr>
<td>10+</td>
<td>$675.00</td>
</tr>
</tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/pald-aav/repcap-plasmids" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="145287">10 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr><tr><td><p><strong class="item_title">pALD-AAV5</strong></p></td><td>5058-10</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">5058-10 10 mg Pricing</strong><div id="pricetable_145299" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody>
<tr>
<td>1-9</td>
<td>$810.00</td>
</tr>
<tr>
<td>10+</td>
<td>$675.00</td>
</tr>
</tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/pald-aav/repcap-plasmids" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="145299">10 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr><tr><td><p><strong class="item_title">pALD-AAV6</strong></p></td><td>5059-10</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">5059-10 10 mg Pricing</strong><div id="pricetable_145311" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody>
<tr>
<td>1-9</td>
<td>$810.00</td>
</tr>
<tr>
<td>10+</td>
<td>$675.00</td>
</tr>
</tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/pald-aav/repcap-plasmids" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="145311">10 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr></tbody></table>
| Appearance | Clear and Colorless |
| Buffer | TE |
| USP Sterility <71> * | No Growth |
| Concentration | 1.0 mg/mL +/- 10% |
| DNA Homogeneity | ≥ 80% Supercoiled |
| Endotoxin | ≤ 100 EU/mg |
| Identity | Confirmed Against DNA Ladder |
| Plasmid Identity | Identical to Reference Sequence |
| ABS 260/280 Ratio Purity | 1.80 - 2.00 |
| Residual Host Genomic DNA via qPCR | ≤ 5% |
| Residual Host Protein via BCA | ≤ 2% |
| Residual Host RNA via SYBR Gold | ≤ 5% by Semi-Quantitative Densitometry |
| Restriction Digest | Matches Expected Restriction Pattern |
| Mycoplasma Contamination | Negative for the Presence of Mycoplasma |
*Sterility testing is not available for research grade material. Bioburden by direct inoculation with a specification of no growth after 48 hours is performed at the research grade level.