Rep/Cap Plasmids

In Stock, Royalty-Free, Packaging Plasmid Solution for Recombinant AAV Manufacturing

pALD-AAV Rep/Cap Plasmids

Recombinant AAV (rAAV) manufacturing is composed of four main parts which include a rep/cap plasmid, a helper plasmid, an expression plasmid, and a viral packaging cell line. Triple transfection of the three plasmids into HEK293 cells is the most frequently used rAAV production method.

Aldevron's pALD-AAV rep/cap plasmids have several advantages:

Royalty free, including for commercial products
Kanamycin resistance
Consistent, reliable supply
Immediately available at research grade

With Aldevron's rep/cap plasmids, clients do not need to manufacture a custom batch as Aldevron maintains each as an in-stock product, reducing timeline and cost.

Each rep/cap plasmid contains the gene sequence for AAV serotype 2 replication (rep) protein paired with either serotype 2, 5 or 6 capsid (cap) protein respectively. Use of AAV serotype 2 rep proteins in combination with other serotypes for cap proteins is referred to as pseudotyping which provides enhanced and refined tissue tropism in some applications.

Plasmid	Rep Serotype	Cap Serotype	Pack Size
pALD-AAV2	2	2	10 mg
pALD-AAV5	2	5	10 mg
pALD-AAV6	2	6	10 mg

Bulk quantities and samples are available upon request.

Plasmid Maps

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<table class="table_order"><thead><tr><th>Description</th><th>Cat #</th><th>Price</th><th>Order</th></tr></thead><tbody><tr><td><p><strong class="item_title">pALD-AAV2</strong></p></td><td>5057-10</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">5057-10 10 mg Pricing</strong><div id="pricetable_145287" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1080.00</td></tr><tr><td>2</td><td>$935.00</td></tr><tr><td>3</td><td>$860.00</td></tr><tr><td>4</td><td>$845.00</td></tr><tr><td>5+</td><td>$804.00</td></tr></tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/pald-aav/repcap-plasmids" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="145287">10 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr><tr><td><p><strong class="item_title">pALD-AAV5</strong></p></td><td>5058-10</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">5058-10 10 mg Pricing</strong><div id="pricetable_145299" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1080.00</td></tr><tr><td>2</td><td>$935.00</td></tr><tr><td>3</td><td>$860.00</td></tr><tr><td>4</td><td>$845.00</td></tr><tr><td>5+</td><td>$804.00</td></tr></tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/pald-aav/repcap-plasmids" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="145299">10 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr><tr><td><p><strong class="item_title">pALD-AAV6</strong></p></td><td>5059-10</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">5059-10 10 mg Pricing</strong><div id="pricetable_145311" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1080.00</td></tr><tr><td>2</td><td>$935.00</td></tr><tr><td>3</td><td>$860.00</td></tr><tr><td>4</td><td>$845.00</td></tr><tr><td>5+</td><td>$804.00</td></tr></tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue=https://www.aldevron.com/products/pald-aav/repcap-plasmids" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="145311">10 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr></tbody></table>

Rep/Cap Plasmids Quality Control Specifications

Appearance	Clear and Colorless
Buffer	TE
USP Sterility <71> *	No Growth
Concentration	1.0 mg/mL +/- 10%
DNA Homogeneity	≥ 80% Supercoiled
Endotoxin	≤ 100 EU/mg
Identity	Confirmed Against DNA Ladder
Plasmid Identity	Identical to Reference Sequence
ABS 260/280 Ratio Purity	1.80 - 2.00
Residual Host Genomic DNA via qPCR	≤ 5%
Residual Host Protein via BCA	≤ 2%
Residual Host RNA via SYBR Gold	≤ 5% by Semi-Quantitative Densitometry
Restriction Digest	Matches Expected Restriction Pattern
Mycoplasma Contamination	Negative for the Presence of Mycoplasma

*Sterility testing is not available for research grade material. Bioburden by direct inoculation with a specification of no growth after 48 hours is performed at the research grade level.

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