Alchemy™ Cell-free DNA Technology

Research Grade Linear DNA Template for mRNA Production

Introducing Aldevron’s new DNA offering - Alchemy cell-free DNA technology. This synthetic DNA technology is a cell-free DNA enzymatic manufacturing process that cuts research grade linear DNA manufacturing time by over 50%, accelerating mRNA development projects with cleaner, safer DNA templates.

Alchemy cell-free DNA technology is now available at research grade to support development and preclinical programs with a future cGMP offering expected to launch in 2025 to support clinical applications.

Why Choose Alchemy™ Cell-free DNA Technology?

Faster Turnaround To Accelerate Your Research

Our Alchemy process significantly reduces production timelines, enabling you to cut your research grade linDNA template production timelines in half.

Embrace the next generation of mRNA manufacturing with Alchemy to expedite your manufacturing timelines and stay ahead in your scientific endeavors.

Traditional Plasmid DNA vs. Alchemy DNA Production Timeline (estimated)

Alchemy DNA reduces manufacturing time by >50%

High Purity DNA Product

Using a synthetic cell-free process, we ensure minimal endotoxin and other host cell-derived impurities. This results in higher purity DNA and mRNA downstream.

Comparable activity to traditional linear DNA templates

Our proprietary cell-free DNA manufacturing process, utilizing rolling circle amplification, supports the rapid production of linear DNA templates.

Alchemy"' cell-free DNA linear templates and traditional plasmid DNA, along with their respective mRNA products, demonstrate comparable integrity. Client data from two different constructs compared linearized plasmid DNA templates with our new cell-free linear DNA offering for mRNA manufacturing. The percent integrity, measured via capillary electrophoresis, showed similar results for both the linear DNA starting materials and the mRNA product after the IVT reaction (Alchemy cell-free DNA template shown in red and plasmid DNA shown in orange).

Comparable Quality Control Specifications to Produce mRNA from pDNA

Alchemy-derived templates meet stringent quality control specifications, ensuring that the mRNA produced is of the highest quality. Here are the detailed specifications:

Attribute	Specs
Formulation	1-5mg/ml
Endotoxin	<2 EU/mg
Residual Protein	<1.00%
Residual Template	<1x10^6 copies/µl

Cleaner and More Efficient

Utilize Alchemy technology for cleaner and more efficient linear templates, ensuring your mRNA is free from contaminants and meets the highest standards of purity and performance.

Frequently asked questions

What quality grades do you have available for cell free linear DNA?

We currently offer Alchemy in linear format for mRNA production at research grade and expect to launch a cGMP offering in 2025.

How has the implementation of this technology accelerated my project's timeline?

The adoption of a synthetic process for enzymatic DNA manufacturing significantly accelerates project timelines by eliminating the need for extensive downstream processing. This streamlined approach reduces the overall manufacturing time and enhances efficiency.

When will linearized Alchemy Cell-free DNA be available at cGMP?

Our linear DNA format is projected to be available in 2025. Please contact us to learn more about manufacturing your construct using Alchemy Cell-free DNA at cGMP.

How does the performance compare to traditional linearized plasmids?

Aldevron partnered with several industry leaders and performed beta testing of our new Alchemy linear DNA offering. During extensive testing, we found its performance and characteristics were comparable to traditional linear DNA templates in both manufacturing and expression performance. To learn more, please contact us about our Alchemy linear DNA services.

Contact Us Today

Ready to enhance your mRNA production process with our Alchemy cell-free DNA? Contact us to learn more and get started.

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