Maximizing mRNA Titers with Codex® HiCap RNA Polymerase
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Explore how RNA titers can be maximized by adjusting reaction conditions to produce the highest quality mRNA to meet your needs
To improve the efficiency of mRNA production workflows, cap analogs have been developed that allow for the addition of a 5’ cap during mRNA synthesis. These co-transcriptional capping methods can produce abundant mRNA bearing a 5’ cap in a single reaction step.
While co-transcriptional capping IVTs are more streamlined than their traditional counterparts, wild-type T7 RNA Polymerase does not efficiently incorporate many of these cap analogs leading to capping efficiencies far below those required for therapeutic use.
The Codex® HiCap RNA Polymerase is an engineered T7 RNA Polymerase designed to address the challenges of efficiently producing therapeutic-grade mRNA at scale. Compared to wild-type T7, Codex® HiCap offers a higher affinity for cap analogs leading to equivalent or better yields and higher capping efficiencies.