Making the Most of pDNA DIY Kits

Considerations when using kits for plasmid DNA production

For unstable plasmids (e.g. AAVs), use recombinant-deficient host strains for plasmid propagation
Be gentle when performing alkaline lysis steps to reduce genomic DNA contamination

Avoid exceeding 25 % of the volume of the growth vessel to ensure maximum aeration
Don’t store biomass in -20 C freezers that undergo thawing cycles
When working with large plasmids (> 20 kb), consider increasing the volumes of the suspension, lysis and neutralization buffers

Our mission is to serve the research community however we can. Even if you prefer DIY plasmid purification, contact us anytime if we can assist with technical problems or anything else. We have a lot of experience and are happy to help. If you are interested in outsourcing your research, contact us to arrange for free prep.